Looking at cartoons (especially animated Disney) is a really good way to get a feel for exaggerated feminine/masculine features, especially if you're going for less realism (which I assumed, since realistic wolves are pretty much monomorphic). Some exambles would be silkier, well kept fur, a slightly smaller nose/muzzle, larger irises/pupils and eyes in general, dark rims around the eyes to give the impression of long lashes (if you overdo that she'll just look halfheartedly goth, though), sometimes a slightly darker, cooler shade of the character's base colour on/near the eyelid can give an eyeshadow-like effect.
You could also use markings and colouration to accentuate more feminine features; softer, curvier lines are percieved as female while sharp edges and straight lines will seem masculine. Even if you already have a character design a little bit of adjustment to lines and marking edges here and there can make a huge difference in how her facial structure/the shape of the mesh is percieved.
